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Natriuretic peptide precursor C coding gene contributes to zebrafish angiogenesis / 生理学报
Acta Physiologica Sinica ; (6): 11-16, 2017.
Article in Chinese | WPRIM | ID: wpr-331598
ABSTRACT
This study aimed to investigate the expression of the natriuretic peptide precursor C coding gene nppc and its role in angiogenesis during embryonic period of the zebrafish. Whole mount in situ hybridization was performed to detect the expression pattern of nppc. nppc specific morpholino and nppc mRNA were injected respectively into the one-cell stage embryo to specifically knock-down and rescue the expression of nppc in Tg (flk1GFP) and Tg (fli1anGFP) transgenic lines. The morphology and endothelial cell number of intersegmental vessel (ISV) were analyzed after imaging using the laser scanning confocal microscope. The results revealed that nppc was expressed in the brain, heart and vasculature of zebrafish larvae at 24 and 48 hours post-fertilization (hpf). Knock-down of nppc affected the development of ISV. Endothelial cell number was reduced after the knock-down of nppc. These results suggest that nppc controls zebrafish angiogenesis by affecting the endothelial cell proliferation and migration.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Physiology / Zebrafish / RNA, Messenger / Animals, Genetically Modified / Cell Movement / Neovascularization, Physiologic / Zebrafish Proteins / Endothelial Cells / Natriuretic Peptides / Cell Proliferation Limits: Animals Language: Chinese Journal: Acta Physiologica Sinica Year: 2017 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Physiology / Zebrafish / RNA, Messenger / Animals, Genetically Modified / Cell Movement / Neovascularization, Physiologic / Zebrafish Proteins / Endothelial Cells / Natriuretic Peptides / Cell Proliferation Limits: Animals Language: Chinese Journal: Acta Physiologica Sinica Year: 2017 Type: Article