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Development of three-dimensional breast cancer cell culture drug resistance model / 生理学报
Acta Physiologica Sinica ; (6): 179-184, 2016.
Article in Chinese | WPRIM | ID: wpr-331668
ABSTRACT
The aim of the present study was to develop three-dimensional (3D) culture model, a more pathologically relevant model, of human breast cancer for drug resistance study. MCF-7 cells were embedded within collagen gel to establish 3D culture model. Cellular morphology was observed using Carmine and HE staining. Cell proliferation was evaluated by CCK-8 assay, and cell activity was detected by Live/Dead staining kit. Drug sensitivities of the 3D culture to doxorubicin, carboplatin, 5-fluorouracil were assayed and compared with those of monolayer (2D) culture. In addition, the levels of drug resistance-related genes P-glycoprotein (P-gp), mrp2 mRNA expressions were detected by real time RT-PCR. Expression level of P-gp protein was detected by Western blot. The results showed that MCF-7 cells in 3D culture formed a number of cell aggregates, and most of them displayed good cell viability. The IC50 values of doxorubicin, carboplatin, 5-fluorouracil were all increased significantly in 3D culture compared with those in 2D culture. Moreover, compared with MCF-7 cells in 2D culture, the cells in 3D culture showed increased mRNA levels of P-gp and mrp2, as well as up-regulated protein expression of P-gp. These results suggest that in vitro collagen-embedded culture system of human breast cancer cells represents an improved pathologically relevant 3D microenvironment for breast cancer cells, providing a robust tool to explore the mechanism of drug resistance of cancer cells.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Breast Neoplasms / Doxorubicin / Cell Survival / ATP Binding Cassette Transporter, Subfamily B, Member 1 / Drug Resistance, Neoplasm / Cell Culture Techniques / Cell Proliferation / MCF-7 Cells Limits: Humans Language: Chinese Journal: Acta Physiologica Sinica Year: 2016 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Breast Neoplasms / Doxorubicin / Cell Survival / ATP Binding Cassette Transporter, Subfamily B, Member 1 / Drug Resistance, Neoplasm / Cell Culture Techniques / Cell Proliferation / MCF-7 Cells Limits: Humans Language: Chinese Journal: Acta Physiologica Sinica Year: 2016 Type: Article