Effects of dahuang zhechong pill on expression and activity of matrix metalloproteinase in rats' hepatic stellate cells / 中国中西医结合杂志
Chinese Journal of Integrated Traditional and Western Medicine
;
(12): 1100-1103, 2005.
Article
in Chinese
| WPRIM
| ID: wpr-331778
ABSTRACT
<p><b>OBJECTIVE</b>To observe the effects of Dahuang Zhechong Pill (DZP) on the gene expression of matrix metalloproteinase-1 (MMP-1) in rats' hepatic stellate cells (HSC) and activity of matrix metalloproteinase-2 (MMP-2) that secreted into the culture base.</p><p><b>METHODS</b>HSC were isolated from the liver of normal rats and incubated with DZP-contained drug serum. The expression of MMP-1 in the HSC was detected by quantitative reverse-transcription polymerase chain reaction (RT-PCR). The activity of MMP-2 was examined by zymography.</p><p><b>RESULTS</b>DZP-contained drug serum could obviously promote the gene expression of MMP-1 in HSC. In the meantime, it could obviously increase the content and activity of MMP-2 synthesized by HSC (P < 0.05).</p><p><b>CONCLUSION</b>The anti-fibrosis action of DZP was correlated to the promotion of HSC's gene expression of MMP-1 and increasing of the contents and activity of MMP-2.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Drugs, Chinese Herbal
/
Random Allocation
/
Rats, Sprague-Dawley
/
Matrix Metalloproteinase 2
/
Matrix Metalloproteinase 1
/
Hepatocytes
/
Cell Biology
/
Genetics
/
Metabolism
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Integrated Traditional and Western Medicine
Year:
2005
Type:
Article
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