Nitric oxide regulates c-fos expression in osteoblastic cells induced by wall-shear / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
;
(6): 479-484, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-332120
ABSTRACT
<p><b>OBJECTIVE</b>To observe regulation of nitric oxide on c-fos expression in osteoblastic cells in response to changes in wall-shear stress in vitro.</p><p><b>METHODS</b>Isolated and purified osteoblastic cells from the calvaria of newborn SD rats were cultured and passaged. The third generation cells, pre-treated with 10% FBS DMEM, 0.3 mmol/L L-NMMA DMEM and 0.1 mmol/L SNP DMEM separately, were subjected to wall-shear stress of 1.2 Pa. Gene expression of the c-fos and NOS activity were studied before (0 min) and 10 min, 15 min, 30 min, 60 min after treated with wall-shear stress.</p><p><b>RESULT</b>The expression of c-fos mRNA was increased transiently after application of 1.2 Pa wall-shear stress in osteoblastic cells and peaked at 15 min. The expression of c-fos mRNA was decreased after pre-application with L-NMMA and increased after use of SNP.</p><p><b>CONCLUSION</b>Changes in the osteoblastic cells mechanical environment may cause a dramatic induction of NO and c-fos expression.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Osteoblasts
/
Skull
/
Stress, Mechanical
/
Biomechanical Phenomena
/
RNA, Messenger
/
Cells, Cultured
/
Proto-Oncogene Proteins c-fos
/
Rats, Sprague-Dawley
/
Nitric Oxide Synthase
/
Cell Biology
Limits:
Animals
Language:
Chinese
Journal:
Journal of Zhejiang University. Medical sciences
Year:
2006
Type:
Article
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