Effect of cinobufacini on vascular contractile of rat thoracic aorta / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
;
(6): 178-181, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-332177
ABSTRACT
<p><b>OBJECTIVE</b>To examine the effect of cinobufacini on rat thoracic aorta and its mechanism.</p><p><b>METHODS</b>Isolated rat thoracic aorta was perfused and isometric tension was recorded by organ bath technique before and after cinobufacini treatment.</p><p><b>RESULT</b>Cinobufacini induced contraction of isolated thoracic aorta with or without endothelium in a concentration-dependent manner (at concentration of 2.5,5.0,7.5,10.0 g/L). The vasoconstriction effect of cinobufacini was more potent in endothelium-denuded aorta ring [(16.3+/-3.39)%, (52.5+/-7.70)%, (60.9+/-8.84)%, (69.2+/-11.34)%] than in endothelium-intact aorta ring [(6.2+/-2.07)%, (14.7+/-4.91), (17.6+/-5.86)%, (20.3+/-6.78)% (P<0.01)]. Its contractile effect was attenuated in Ca(2+)-free solution (about 1/10 of that in buffer with 1.25 mmol/L CaCl(2)) or by the treatment with verapamil (10(-7)mol/L), an L-type calcium channel antagonist. Cinobufacini induced contraction on the endothelium-intact rat aorta was augmented by pretreatment with L-NAME (10(-4)mol/L), a nitric oxide synthase inhibitor.</p><p><b>CONCLUSION</b>Cinobufacini contracts rat thoracic aorta by opening the voltage-dependent Ca(2+) channel and increasing Ca(2+) influx into vascular smooth muscle. Cinobufacini can also stimulate the release of vascular relaxant factor, nitric oxide, from the endothelium and thus antagonize cinobufacini-induced contraction.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Aorta, Thoracic
/
Pharmacology
/
Vasoconstriction
/
Vasoconstrictor Agents
/
In Vitro Techniques
/
Bufanolides
/
Endothelium, Vascular
/
Rats, Sprague-Dawley
/
Metabolism
/
Nitric Oxide
Limits:
Animals
Language:
Chinese
Journal:
Journal of Zhejiang University. Medical sciences
Year:
2006
Type:
Article
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