Relation between mutant p53 and multidrug resistance in gastric cancer / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
;
(6): 91-98, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-332193
ABSTRACT
<p><b>OBJECTIVE</b>To explore the relationship between mutant p53 and multidrug resistance in gastric cancer.</p><p><b>METHODS</b>Mutant p53 (mp53) and mp53+sv40Tag were transferred to gastric cancer cell line SGC-7901. The MDR-1 mRNA was examined using RT-PCR, and the difference in chemotherapeutic sensitivity of SGC-7901 cells with mutant p53 was compared with those with mp53+sv40Tag and controls by MTT method.</p><p><b>RESULTS</b>SGC-7901 cells with mutant p53 showed higher MDR-1 mRNA than that of other two groups. SGC-7901 cells with mutant p53 showed higher chemotherapeutic sensitivity to 5-Fu than that with mp53+sv40Tag and control (P<0.05), but no difference between those with mp53+sv40Tag and control (P>0.05). SGC-7901 cells with mutant p53 and those with mp53+sv40Tag showed higher chemotherapeutic sensitivity to ADM than control (P<0.05), but no difference between those with mp53 and with mp53+sv40Tag (P>0.05). There was no difference in chemotherapeutic sensitivity of SGC-7901 cells with mutant p53 compared with those with mp53+sv40Tag and control to CDDP (P>0.05).</p><p><b>CONCLUSION</b>Mutante p53 genes relates to multidrug resistance of gastric cancer.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Stomach Neoplasms
/
RNA, Messenger
/
Tumor Cells, Cultured
/
Adenocarcinoma
/
Tumor Suppressor Protein p53
/
ATP Binding Cassette Transporter, Subfamily B, Member 1
/
Drug Resistance, Multiple
/
Genetics
/
Mutation
Limits:
Humans
Language:
Chinese
Journal:
Journal of Zhejiang University. Medical sciences
Year:
2006
Type:
Article
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