Using 1H-MRS to evaluate the effects of warm ischemia reperfusion injury on the regeneration of hepatic cells in orthotopic transplanted livers in rats / 中华肝脏病杂志
Chinese Journal of Hepatology
;
(12): 274-278, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-332258
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the prognostic value of proton magnetic resonance spectroscopy (1H-MRS) in the study of warm ischemia reperfusion injury to the regeneration of hepatic cells of the livers following their orthotopic transplantation in rats.</p><p><b>METHODS</b>A rat orthotopic liver transplantation (OLT) model with warm ischemia, the experimental group, was established and the same was done with a control group but without warm ischemia of the livers. They were studied at 6 time points (6 hours, 1, 3, 7, 14, and 30 days after OLT). All rats took axial T1 weighted and T2 weighted imaging scans and 1H MR spectroscopies.</p><p><b>RESULTS</b>The positive rate of proliferating cell nuclear antigen (PCNA) and the mean peak choline/water ratio in the experimental group were significantly higher than those in the control group and the peak choline /water ratio had a positive correlation with the positive rate of PCNA. Serum ALT and AST increased significantly after OLT, especially during the 6 hour to 3day period. The levels of ALT and AST were markedly higher in the experimental group compared to the control group.</p><p><b>CONCLUSION</b>Warm ischemia reperfusion injury of OLT has a significant effect on the regeneration of hepatic cells, and the choline peak of 1H-MRS can be used to evaluate the regeneration of hepatic cells non-invasively.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Magnetic Resonance Spectroscopy
/
Reperfusion Injury
/
Liver Transplantation
/
Rats, Sprague-Dawley
/
Hepatocytes
/
Cell Biology
/
Diagnosis
/
Warm Ischemia
/
Graft Survival
/
Liver Regeneration
Type of study:
Diagnostic study
/
Prognostic study
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Hepatology
Year:
2008
Type:
Article
Similar
MEDLINE
...
LILACS
LIS