In vitro expansion of T cells stimulated by combination of IL-2, IL-7 and IL-15 / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 1590-1594, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-332313
ABSTRACT
The aim of this study was to compare cell proliferation and function of the T cells acquired under various culture conditions for establishing a simple, safe and efficient cell expansion protocol in vitro. The peripheral blood mononuclear cells (PBMNC) were isolated and stimulated with autologous dendritic cells (DC) and EBV-transformed B lymphoblastoid cell line (BLCL) weekly. The cell proliferation test, flow cytometry with PI and Annexin V double staining, Cr release test and ELISPOT test were used to detect the cell expansion level, frequency of IFN-γ producing T cells, killing activity of antigen-specific T cells, cell apoptotic status and cell differentiation potential, respectively. The results indicated that use of IL-2 combined with IL-7 and IL-15 resulted in the highest cell expansion comparing to the use of IL-2 alone and the use of CD3/28 Microbeads. Also the cells obtained under cultivating with IL-2, IL-7 and IL-15 together showed high frequency of IFN-γ producing cells, strong killing activity, high viability and high differentiation potential with large portion of CD3(+)CD8(+) population among the T cells. It is concluded that a protocol is established in which the use of IL-2 combined with IL-7 and IL-15 induces the biggest cell expansion, expanded cells show high viability, strong differentiation potential, high frequency of IFN-γ producing cells and strong killing activity.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Dendritic Cells
/
Cell Line, Transformed
/
T-Lymphocytes
/
Cell Separation
/
Interleukin-7
/
Interleukin-2
/
Interleukin-15
/
Cell Biology
/
Cell Proliferation
Limits:
Humans
Language:
Chinese
Journal:
Journal of Experimental Hematology
Year:
2010
Type:
Article
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