Effect of valproic acid on apoptosis of leukemia HL-60 cells and expression of h-tert gene / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 1445-1450, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-332341
ABSTRACT
This study was aimed to clarify whether valproic acid (VPA) induces apoptosis of leukemia HL-60 cell line and its possible mechanism. The effect of different concentrations and treatment time of VPA on HL-60 cell proliferation was assayed by cytotoxicity test (CCK-8 method) and fluorescence microscopy, and flow cytometry was used to detect cell apoptosis. The expressions of telomerase subunit h-tert mRNA and apoptosis-related protein as well as caspase-3 activity were detected by real time-quantitative PCR, Western blot and ELISA respectively. The results indicated that VPA inhibited proliferation of HL-60 cells and induced cell apoptosis in a dose dependent manner (r = -0.87). The expressions of anti-apoptotic protein BCL-2 and h-tert mRNA were significantly decreased while the pro-apoptotic protein BAX and caspase-3 activity increased after treatment with VPA. The apoptosis rate of HL-60 cell was negatively correlated with expression of h-tert mRNA. It is concluded that VPA can inhibit leukemia HL-60 cell proliferation and induce apoptosis. The VPA displays anti-leukemia activity possibly through reducing h-tert mRNA and BCL-2 protein expression, increasing BAX expression and activity of caspase-3.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Valproic Acid
/
Apoptosis
/
Telomerase
/
HL-60 Cells
/
Proto-Oncogene Proteins c-bcl-2
/
Bcl-2-Associated X Protein
/
Caspase 3
/
Metabolism
Limits:
Humans
Language:
Chinese
Journal:
Journal of Experimental Hematology
Year:
2010
Type:
Article
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