Construction of human metapneumovirus DNA vaccine and study on its immune response in mice / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 100-102, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-332416
ABSTRACT
<p><b>OBJECTIVE</b>To construct human metapneumovirus (hMPV) DNA vaccines and evaluate the cellular and humoral immune response in mice.</p><p><b>METHODS</b>Fusion protein FdeltaTM (without transmembrane domain) gene and M gene of hMPV were amplified from cDNA by PCR, then DNA vaccines pcDNA3.1His-FdeltaTM and pcDNA3.1His-M were constructed to verify the expression of F and M protein by Western blotting and indirect immunofluorescent assay (IFA) respectively. Serum IgG and spleen cell CTL were detected with ELISA and ELISPOT assay after the BALB/c mice were immunized intramuscularly with the vaccines.</p><p><b>RESULTS</b>The candidate DNA vaccines could express FdeltaTM and M protein as detected with Western blotting and IFA. The IgG antibody titers of mice was 144 when immunized with pcDNA3.1His-FdeltaTM, but could increase to 164 when co-immunized with pcDNA3.1His-M. ELISPOT assay demonstrated that IFN-gamma-secreting effector T cells reached 42 +/- 8.9 in co-immunization group, higher than single vaccine pcDNA3.1His-FdeltaTM group (32 +/- 7.4).</p><p><b>CONCLUSION</b>DNA vaccine pcDNA3.1His-FdeltaTM could induce specific cellular and humoral immune responses, and the immune response could increase when co-immunization with pcDNA3.1His-M was carried out.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Viral Proteins
/
Virology
/
Blood
/
Viral Vaccines
/
Immunization
/
Paramyxoviridae Infections
/
Vaccines, DNA
/
Metapneumovirus
/
Allergy and Immunology
/
Genetics
Limits:
Animals
/
Female
/
Humans
Language:
Chinese
Journal:
Chinese Journal of Experimental and Clinical Virology
Year:
2009
Type:
Article
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