Application of 293 cells in in vitro detection of carcinogens / 中华实验和临床病毒学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the application of 293 cells to detect suspected carcinogens and provide experimental evidence by using in vitro cell transformation assay and tumorigenicity study.</p><p><b>METHODS</b>The transformation systems of cells cultured in vitro have been adopted to clarify the tumor promotive activity of Microcystin LR (MC-LR). The malignant transformation of 293 cells induced by MC-LR is tested by several methods including clone forming in soft agarose, serum requirement assay and tumor forming in mice to define the promotive activity of 293 cells.</p><p><b>RESULTS</b>293 cell acted like tumor cells after induced by MC-LR serum dependence decreased, anchorage independence growth in soft agarose and formed cell clones, malignant tumors appeared in SCID mice.</p><p><b>CONCLUSION</b>293 cells were easy to culture and sensitive to environmental carcinogens so that can be used in detection of suspicious carcinogens.</p>