Construction and characterization of reverse genetic system for H9N2 avian influenza / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 41-43, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-332435
ABSTRACT
<p><b>OBJECTIVE</b>To provide a technology platform for vaccine development as well as the research on transmission and pathogenesis, the reverse genetic system for H9N2 avian influenza virus was established.</p><p><b>METHODS</b>Eight full-length cDNAs of avian influenza virus A/Guangzhou/333/99 (H9N2) were amplified by RT-PCR and separately cloned into the transcription/expression vector, pCI-polI. The 8 plasmids DNA was cotransfected into 293T cell, the cell supernatant was collected and inoculated into embryonated eggs, the rescued virus from the allantoic fluid was identified by hemagglutinination assay.</p><p><b>RESULTS</b>The avian influenza H9N2 virus was successfully rescued by 8 plasmids co-transfection in 293T cells. The hemagglutinination titer of the rescued virus is up to 2(9)/50 microl and its growth curve remained relatively as to the wild-type virus.</p><p><b>CONCLUSION</b>The reverse genetic for avian influenza H9N2 subtype virus has been established successfully.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Physiology
/
Plasmids
/
Virology
/
Genetic Engineering
/
Cell Line
/
Influenza, Human
/
Influenza A Virus, H9N2 Subtype
/
Genetic Vectors
/
Genetics
/
Methods
Limits:
Animals
/
Female
/
Humans
Language:
Chinese
Journal:
Chinese Journal of Experimental and Clinical Virology
Year:
2009
Type:
Article
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