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The infection of dendritic cells by recombinant adenoviral vector carrying HBsAg-HSP70 chimeric gene and its biological characteristics observations / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology ; (6): 29-31, 2009.
Article in Chinese | WPRIM | ID: wpr-332439
ABSTRACT
<p><b>OBJECTIVE</b>To test the infeciton efficiency of recombinant adenoviral vector carrying HBsAg-HSP70 chimeric gene and to abserve its biological characteristics.</p><p><b>METHODS</b>Peripheral blood mononuclear cells (PBMC) were separated from healthy blood donor and they were infected by Ad-HSP70-HBsAg on the first day of isolation. DCs were induced in medium with cytokines IL-4, GM-CSF and TNF-alpha in vitro. The biological characteristics of DC induced were analyzed by inverted fluorecent microscope, RT-PCR, flow cytometer (FACS), and mixed lymphocyte reaction (MLR).</p><p><b>RESULTS</b>The traced gene-GFP were abserved in DCs by inverted fluorecent microscope and HSP70-HBsAg gene mRNA expression was detected by RT-PCR after the Ad-HSP70-HBsAg infection. FACS analysis shown that the expression of CD1a, CD80, CD86 and HLA-DR on surfece of two groups of DCs were similar. MLR showed that there are not a statitic difference of stimulated index (SI) between two groups.</p><p><b>CONCLUSION</b>Results indicated that Ad-HSP70-HBsAg can effectively infected DCs without affecting its biological characteristics.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Physiology / Virology / Dendritic Cells / Recombinant Fusion Proteins / Cells, Cultured / Adenoviridae / Cytokines / Lymphocyte Culture Test, Mixed / HSP70 Heat-Shock Proteins / Allergy and Immunology Limits: Humans Language: Chinese Journal: Chinese Journal of Experimental and Clinical Virology Year: 2009 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Physiology / Virology / Dendritic Cells / Recombinant Fusion Proteins / Cells, Cultured / Adenoviridae / Cytokines / Lymphocyte Culture Test, Mixed / HSP70 Heat-Shock Proteins / Allergy and Immunology Limits: Humans Language: Chinese Journal: Chinese Journal of Experimental and Clinical Virology Year: 2009 Type: Article