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Atomic force microscopy investigation of the denudation of the envelope of influenza virus treated with Nonidet P-40 / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology ; (6): 507-509, 2008.
Article in Chinese | WPRIM | ID: wpr-332451
ABSTRACT
<p><b>OBJECTIVE</b>Through observing the morphology and topography of the prepared influenza viruses (H1N1) treated with the different Nonidet P-40 solutions using atomic force microscopy (AFM), to explore the application of AFM on the research of the internal character of viral morphology and structural virology.</p><p><b>METHODS</b>The virus samples were treated with serial diluted Nonidet P-40 solutions from 0.05% to 0.20% and then investigated by AFM with the tapping mode in air at room temperature to obtain the morphology and topography changes including height data,amplitude data and phase data for both spherical and filamentous influenza virus A.</p><p><b>RESULTS</b>The serial AFM images show that the erosion degree of the virions is proportional with the improvement of NP-40 concentration,and partly denuded virion image appeared at 0.05% NP-40 treatment, which was revealed clearly on both amplitude images and phase images.</p><p><b>CONCLUSION</b>This work demonstrated for the first time that the internal topography of influenza virion could be revealed by AFM via suitable nonionic surfactants chemical dissection.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Orthomyxoviridae / Influenza A virus / Pharmacology / Polyethylene Glycols / Spermatocidal Agents / Chemistry / Microscopy, Atomic Force / Nanostructures Limits: Humans Language: Chinese Journal: Chinese Journal of Experimental and Clinical Virology Year: 2008 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Orthomyxoviridae / Influenza A virus / Pharmacology / Polyethylene Glycols / Spermatocidal Agents / Chemistry / Microscopy, Atomic Force / Nanostructures Limits: Humans Language: Chinese Journal: Chinese Journal of Experimental and Clinical Virology Year: 2008 Type: Article