Molecular characterization and sequence comparison of the M and S segments of Hantavirus ZJ5 strain / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
; (6): 434-436, 2008.
Article
in Zh
| WPRIM
| ID: wpr-332475
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>In order to understand the molecular characters of Hantavirus ZJ5 strain, its complete M and S genome were sequenced and compared with that of other hantavirus strains.</p><p><b>METHODS</b>We prepared the total RNA from ZJ5. Infected cells and the raw or purified RT-PCR product was cloned and sequenced.</p><p><b>RESULTS</b>With sequence compation, we found ZJ5 strain complete M and S segment had higher homology with SEO-type strains than other type of HV, but differential genes were 11.7%-19.2% and 6.7%-14.5% from SEOV. The phylogenetic trees constructed by complete M ind S segment showed that ZJ5 strain was located in SEOV group, and structured alone embranchment.</p><p><b>CONCLUSION</b>The ZJ5 strain was believed to belong to SEO-type virus,and suggest that ZJ5 strain is a new subtype S SEOV group,and structured alone embranchment.</p><p><b>CONCLUSION</b>The ZJ5 strain was believed to belong to SEO-type virus, and suggest that ZJ5 strain is a new subtype from other SEO viruses.</p>
Full text:
1
Index:
WPRIM
Main subject:
Phylogeny
/
Virology
/
DNA, Viral
/
Molecular Sequence Data
/
Base Sequence
/
Amino Acid Sequence
/
Minor Lymphocyte Stimulatory Loci
/
Orthohantavirus
/
Sequence Analysis, DNA
/
Reverse Transcriptase Polymerase Chain Reaction
Language:
Zh
Journal:
Chinese Journal of Experimental and Clinical Virology
Year:
2008
Type:
Article