Application of a simple method for the detection of measles virus genome by loop-mediated isothermal amplification (LAMP) / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
; (6): 403-405, 2008.
Article
in Zh
| WPRIM
| ID: wpr-332485
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>A new simple RT-LAMP method was applied to detect measles virus nucleic acid and compared with nest-RT-PCR.</p><p><b>METHODS</b>Compare the detection rate of the RT-LAMP method with that of nest-RT-PCR by detecting measles virus nucleic acid from measles virus and clinical samples.</p><p><b>RESULTS</b>The nucleic acid positive rates of all 23 strains of measles virus are all 100% by the two methods. But to the detection of 18 clinical samples which are negative in measles isolation, the nest-RT-LAMP showed 56.52% positive rate of nucleic acid of measles virus and nest-RT-PCR showed 47.83%.</p><p><b>CONCLUSION</b>RT-LAMP is more sensitive than nest-RT-PCR.</p>
Full text:
1
Index:
WPRIM
Main subject:
Virology
/
Genome, Viral
/
Reverse Transcriptase Polymerase Chain Reaction
/
Measles
/
Measles virus
/
Methods
Type of study:
Diagnostic_studies
Limits:
Humans
Language:
Zh
Journal:
Chinese Journal of Experimental and Clinical Virology
Year:
2008
Type:
Article