Change of IL-22R1 expression in human airway smooth muscle cells in response to different stimulating agents / 南方医科大学学报
Journal of Southern Medical University
;
(12): 979-982, 2011.
Article
in Chinese
| WPRIM
| ID: wpr-332503
ABSTRACT
<p><b>OBJECTIVE</b>To explore the effects of serum of asthmatic patients, dexamethasone, interleukin-4 (IL-4), interferon-gamma (IFN-γ) and transforming growth factor-β (TGF-β) on the expression of interleukin-22 receptor 1 (IL-22R1) mRNA and protein in HASMCs in vitro.</p><p><b>METHODS</b>IL-22R1 mRNA and protein expressions in HASMCs treated with different stimulating agents were measured by real-time PCR and Western blotting, respectively.</p><p><b>RESULTS</b>IL-22R1 mRNA and protein expressions in HASMCs were significantly increased after stimulation by serum from asthmatic patients, but decreased after co-stimulation with dexamethasone. IL-22R1 mRNA and protein expressions in the cells both increased after stimulation by IL-4, IFN-γ and TGF-β.</p><p><b>CONCLUSION</b>IL-22R1 in HASMCs might be involved in the pathogenesis of asthma, and the therapeutic effect of dexamethasone on asthma is mediated, at least partially, by IL-22R1. The effects of IFN-γ, IL-4, and TGF-β on asthma may also be attributed to their actions on HASMCs.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Asthma
/
Blood
/
RNA, Messenger
/
Cell Line
/
Transforming Growth Factor beta
/
Interleukin-4
/
Interferon-gamma
/
Receptors, Interleukin
/
Myocytes, Smooth Muscle
Limits:
Humans
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2011
Type:
Article
Similar
MEDLINE
...
LILACS
LIS