Localization and expression of Slingshot-1L in peripheral eosinophils from patients with acute asthma exacerbation / 南方医科大学学报
Journal of Southern Medical University
;
(12): 928-932, 2011.
Article
in Chinese
| WPRIM
| ID: wpr-332513
ABSTRACT
<p><b>OBJECTIVE</b>Eosinophils play a pivotal role in asthmatic airway inflammation. We previously found a significantly high expression of Slingshot-1L (SSH-1L) in peripheral eosinophils in acute exacerbations of asthma. Objective To investigate the expression and localization patterns of SSH-1L in peripheral blood eosinophils of asthmatic patients and their changes after treatment with inhaled corticosteroids.</p><p><b>METHODS</b>We recruited 4 outpatients with acute exacerbations of asthma who received no previous corticosteroid treatment and 1 healthy volunteer. From all the subjects 30 ml peripheral venous blood samples were collected before and after a 3-month treatment with inhaled fluticasone. The eosinophils were isolated, purified and counted, and the expressions of SSH-1L in the eosinophils were examined by RT-PCR and Western blotting. The localization of SSH-1L phosphatases in the peripheral eosinophils was detected by immunofluorescence assay in one patient.</p><p><b>RESULTS</b>SSH-1L phosphatases distributed diffusely in the cytoplasm, especially dense near the membrane of the peripheral eosinophils. Glucocorticoids treatment resulted in a significant reduction in both the SSH-1L mRNA expression (0.7403∓0.1124 vs 0.4101∓0.0363, P=0.001) and SSH-1L protein expression (0.3410∓0.1337 vs 0.1543∓0.0551, P=0.039).</p><p><b>CONCLUSION</b>A high expression of SSH-1L in peripheral eosinophils in acute exacerbations of asthma may play a role in the activation and migration of eosinophils. The efficacy of inhaled corticosteroids in asthma control might be partly attributed to a down-regulated expression of SSH-1L.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Asthma
/
Blood
/
Phosphoprotein Phosphatases
/
Therapeutic Uses
/
Drug Therapy
/
Eosinophils
/
Glucocorticoids
/
Metabolism
Limits:
Adult
/
Aged
/
Female
/
Humans
/
Male
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2011
Type:
Article
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