Determination of the content of phloridzin using reversed-phase high-performance liquid chromatography / 南方医科大学学报

ABSTRACT

<p><b>OBJECTIVE</b>To establish a reversed-phase high-performance liquid chromatographic (RP-HPLC) method for determination of phloridzin content.</p><p><b>METHODS</b>A RP-HPLC method was established for determination of phloridzin using an Inertsil ODS-3 (4.6×150 mm, 5 µm) column with the detection wavelength of 288 nm, flow rate of 1.0 ml/min, and column temperature of 25 degrees celsius;.</p><p><b>RESULTS</b>The result showed that the phloridzin had a good linear relationship when its concentration ranged between 0.5988 and 89.72 µg/ml. The regression equation was Y=46.370 X-0.6728 (r=0.9999, n=3). The average recovery of phloridzin was 99.40% with the relative standard deviations (RSD) of 0.67%.</p><p><b>CONCLUSION</b>This method is simple, quick and accurate for determination of phloridzin content.</p>