Salidroside inhibits clinorotation-induced apoptosis in pulmonary microvascular endothelial cells / 南方医科大学学报
Journal of Southern Medical University
;
(12): 649-652, 2011.
Article
in Chinese
| WPRIM
| ID: wpr-332582
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the inhibitory effect of salidroside (Sal) on pulmonary microvascular endothelial cell (HPMEC) apoptosis induced by simulated microgravity and its mechanism.</p><p><b>METHODS</b>Human pulmonary microvascular endothelial cells cultured in vitro were divided into control group, clinorotation group and clinorotation+Sal pretreatment groups. Microgravity was simulated by clinorotation. The apoptotic rate of HPMECs was detected by flow cytometry using Annexin V-FITC staining, and the expressions of bcl-2, bax, and caspase-3 at the mRNA and protein levels were determined by real-time PCR and Western blotting, respectively.</p><p><b>RESULTS</b>A 72-h clinorotation significantly induced apoptosis in HPMECs. Real-time PCR results demonstrated a significantly lowered bcl-2 but increased bax and caspase-3 mRNA expressions in clinorotation group as compared with those in the control group. Western blotting showed that clinorotation inhibited the protein expressions of PI3K and p-AKT and increased caspase-3 protein expression. Salidroside significantly inhibited the cell apoptosis, reversed the expressions of Bcl-2 and Bax, and attenuated the decrease in the protein expression of PI3K and phosphorylation level of AKT. Salidroside also antagonized the activation of caspase-3.</p><p><b>CONCLUSION</b>PI3K/AKT pathway and caspase 3 are involved in the apoptosis of HPMVECs induced by clinorotation, and the effect of clinorotation can be reversed by salidroside, suggesting the potential value of salidroside for application in spaceflight.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Phenols
/
Weightlessness
/
Endothelium, Vascular
/
Signal Transduction
/
Cell Line
/
Apoptosis
/
Cell Biology
/
Endothelial Cells
/
Caspase 3
Limits:
Humans
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2011
Type:
Article
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