Expression of endothelial nitric oxide synthase mRNA in human RBCs during storage / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 481-483, 2013.
Article
in Chinese
| WPRIM
| ID: wpr-332753
ABSTRACT
This study was purposed to identify endothelial nitric oxide synthase (eNOS) mRNA in human RBCs during storage and to investigate the relationship of its changing profile and preservation time at 4°C. RT-PCR and gene sequencing were applied to identify eNOS-mRNA in banked RBC. Real time PCR was used to study the relationship of eNOS-mRNA expression and preservation time. The results showed that eNOS mRNA was detected in RBC. Compared with fresh RBC, the content of eNOS mRNA in RBC was 0.868 ± 0.119 stored for 1 week, which was 0.379 ± 0.289, 0.108 ± 0.134, 0.141 ± 0.141, 0.125 ± 0.12 stored for 2, 3, 4 and 5 weeks respectively. It is concluded that eNOS mRNA exists in human RBC and its content is decreasing gradually along with the prolongation of storage time in banked RBC. Stored for 3 weeks, the content of eNOS-mRNA remains to be at lower level of concentration in human RBC.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Blood Donors
/
Blood Preservation
/
RNA, Messenger
/
Erythrocytes
/
Nitric Oxide Synthase Type III
/
Genetics
/
Metabolism
Limits:
Humans
Language:
Chinese
Journal:
Journal of Experimental Hematology
Year:
2013
Type:
Article
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