Expression of SALL4 gene in patients with acute and chronic myeloid leukemia / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 315-319, 2013.
Article
in Zh
| WPRIM
| ID: wpr-332789
Responsible library:
WPRO
ABSTRACT
The aim of this study was to investigate the expression level of the SALL4 gene and its clinical significance in patients with acute myeloid leukemia (AML) and chronic myeloid leukemia (CML). Real-time quantitative PCR (RQ-PCR) was performed to detect the expression level of SALL4 mRNA in bone marrow mononuclear cells (BMMNC) from 35 AML, 12 CML patients and 24 iron deficiency anemia patients as controls. The results indicated that the expression level of SALL4 in AML (0%-14%, median 1.43%) was obviously higher than that in controls (0% - 1%, median 0%) (P < 0.001). SALL4 expression was positive in 65.7% (23/35) AML patients. The frequency of SALL4 expression was in M2 (86.7%, 13/15) > M3 (75.0%, 6/8) > M1 (60.0%, 3/5) > M4 (14.3%, 1/7), and the difference among 4 groups was statistically significant (P = 0.008); there was no correlation of the frequency of SALL4 expression with the age, sex, white blood cell WBC count, hemoglobin concentration, platelet count and chromosomal abnormalities of AML patients (P > 0.05). All the 13 CML cases showed positive expression of SALL4 gene (1% - 128%, median 19.39%), which was higher than that in controls (P < 0.001). The analysis of receiver operating characteristic (ROC) curve showed the area under ROC curve (AUC) of AML and CML were 0.983 (95% confidence interval: 0.95 - 1.017) and 0.997 (95% confidence interval: 0.986 - 1.007) respectively. It is concluded that SALL4 expression is a common molecular event and can be considered as a molecular marker for assisting diagnosis of AML and CML.
Full text:
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Index:
WPRIM
Main subject:
Transcription Factors
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Leukemia, Myelogenous, Chronic, BCR-ABL Positive
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Leukemia, Myeloid, Acute
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Case-Control Studies
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Gene Expression
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Genetics
Type of study:
Observational_studies
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Prognostic_studies
Limits:
Adolescent
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Adult
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Aged
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Aged80
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Female
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Humans
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Male
Language:
Zh
Journal:
Journal of Experimental Hematology
Year:
2013
Type:
Article