Cloning of differentially expressed genes following lipopolysaccharide stimulation in human umbilical vein endothelial cells / 中华创伤杂志(英文版)
Chinese Journal of Traumatology
; (6): 107-113, 2003.
Article
in En
| WPRIM
| ID: wpr-332908
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To clone the differentially expressed genes in human umbilical vein endothelial cells (HUVEC) stimulated by lipopolysaccharide (LPS).</p><p><b>METHODS</b>Two-directional (forward and backward) suppression subtractive hybridization (SSH) was performed on HUVEC cultured in either standard media or treated for 6 hours with LPS (100 ng/ml). To restrict the number of false-positive clones, colony dot hybridization was used to further verify the differentially expressed cDNA clones. Positive clones were sequenced.</p><p><b>RESULTS</b>These analyses have identified both novel and known genes whose expression is influenced by LPS. The known genes include a group related to proinflammatory events, a group related to cellular apoptosis and proliferation, a group related to protein synthesis and cytoskeletal rearrangment, and a group related to energy metabolism and signal transduction.</p><p><b>CONCLUSIONS</b>SSH is a powerful technique of high sensitivity for the detection of differential gene expression in HUVEC stimulated by LPS.</p>
Full text:
1
Index:
WPRIM
Main subject:
Pharmacology
/
Umbilical Veins
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Endothelium, Vascular
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Gene Expression
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Cells, Cultured
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Lipopolysaccharides
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Polymerase Chain Reaction
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Cloning, Molecular
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DNA, Complementary
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Genetics
Limits:
Humans
Language:
En
Journal:
Chinese Journal of Traumatology
Year:
2003
Type:
Article