Activation of NF-kappaB and apoptosis of intestinal epithelial cells induced by hydrogen peroxide / 中华创伤杂志(英文版)
Chinese Journal of Traumatology
;
(6): 209-213, 2002.
Article
in English
| WPRIM
| ID: wpr-332966
ABSTRACT
<p><b>OBJECTIVE</b>In vitro model of hydrogen peroxide induced apoptosis of SW-480 cells was used to investigate the role of NF-kappaB in the pathogenesis of reactive oxygen species induced apoptosis of intestinal epithelial cells.</p><p><b>METHODS</b>Ultra-structural changes were observed. Apoptosis of SW-480 cell line was determined by Annexin-V and PI double-stained flow cytometry. Nuclear translocation of NF-kappaB was determined by anti-NF-kappaB polyclonal antibody and EB double-staining. NF-kappaB activity was studied by electrophoretic mobility shift assays. RT-PCR was performed to study expression of NF-kappaB mRNA.</p><p><b>RESULTS</b>Hydrogen peroxide led to apoptosis of SW-480 cells, condensed or semilunar chromatin even apoptotic bodies could be observed. Nuclear translocation of NF-kappaB, increase of NF-kappaB activity and expression of NF-kappaB mRNA were found simultaneously.</p><p><b>CONCLUSIONS</b>Early activation of NF-kappaB may be one of the mechanisms of apoptosis in intestinal epithelial cells by reactive oxygen species.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Tumor Cells, Cultured
/
NF-kappa B
/
Reactive Oxygen Species
/
Apoptosis
/
Microscopy, Confocal
/
Toxicity
/
Hydrogen Peroxide
/
Intestinal Mucosa
/
Metabolism
Type of study:
Prognostic study
Limits:
Humans
Language:
English
Journal:
Chinese Journal of Traumatology
Year:
2002
Type:
Article
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