Protective effect of hemoglobin-induced heme oxygenase-1 on injured lungs caused by limb ischemia-reperfusion in rats / 中华创伤杂志(英文版)
Chinese Journal of Traumatology
;
(6): 86-91, 2002.
Article
in English
| WPRIM
| ID: wpr-332993
ABSTRACT
<p><b>OBJECTIVE</b>To determine the role of hemoglobin (HB) induced heme oxygenase-1 (HO-1) in injured lungs caused by limb ischemia-reperfusion (I/R) in rats.</p><p><b>METHODS</b>A rat model of ischemia in the hind limbs was made by clamping the infrarenal aorta with a microvascular clip, and lung injury occurred after reperfusion. To induce the expression of HO-1 in the lungs, Hb was administrated intraperitoneally at 16 hours before reperfusion. Northern blotting and Western blotting were used to detect the expression of HO-1 in the lungs, and the carboxyhemoglobin (COHb) level in arterial blood was assayed. The effect of hemoglobin (Hb) on the injured lungs after limb I/R was determined by measuring the changes of lung histology, polymorphonuclear (PMN) count, malondialdehyde (MDA) content and wet-to-dry weight ratio (W/D). Zinc protoporphyrin (ZnPP), an inhibitor of HO, was used to determine whether HO-1 was induced by Hb after lung injury.</p><p><b>RESULTS</b>Hb led to a significant increase in HO-1 mRNA and protein expression in the lungs, accompanied by the increase of COHb level in arterial blood. Compared with the sham controls, the lung PMN count, MDA content and W/D significantly increased at 4 hours after limb I/R, which reversed by the pretreatment with Hb at 16 hours before reperfusion. ZnPP blocked this protective role of Hb in the injured lungs.</p><p><b>CONCLUSIONS</b>Hb can induce the lung HO-1 expression, which plays an important role in the defense against I/R induced lung injury in rats.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Reference Values
/
Carboxyhemoglobin
/
Hemoglobins
/
RNA, Messenger
/
Reperfusion Injury
/
Blotting, Western
/
Blotting, Northern
/
Probability
Type of study:
Diagnostic study
Limits:
Animals
Language:
English
Journal:
Chinese Journal of Traumatology
Year:
2002
Type:
Article
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