Blocking transforming growth factor-beta receptor signaling down-regulates transforming growth factor-beta1 autoproduction in keloid fibroblasts / 中华创伤杂志(英文版)
Chinese Journal of Traumatology
; (6): 77-81, 2002.
Article
in En
| WPRIM
| ID: wpr-332995
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study transforming growth factor-beta1 (TGF-beta1) autoproduction in keloid fibroblasts and the regulation effect of blocking TGF-beta intracellular signaling on rhTGF-beta1 autoproduction.</p><p><b>METHODS</b>Keloid fibroblasts cultured in vitro were treated with either rhTGF-beta1 (5 ng/ml) or recombinant adenovirus containing a truncated type II TGF-beta receptor gene (50 pfu/cell). Their effects of regulating gene expression of TGF-beta1 and its receptor I and II were observed with Northern blot.</p><p><b>RESULTS</b>rhTGF-beta1 up-regulated the gene expression of TGF-beta1 and receptor I, but not receptor II. Over-expression of the truncated receptor II down-regulated the gene expression of TGF-beta1 and its receptor I, but not receptor II.</p><p><b>CONCLUSIONS</b>TGF-beta1 autoproduction was observed in keloid fibroblasts. Over-expression of the truncated TGFbeta receptor II decreased TGF-beta1 autoproduction via blocking TGF-beta receptor signaling.</p>
Full text:
1
Index:
WPRIM
Main subject:
Pharmacology
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RNA, Messenger
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Signal Transduction
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Down-Regulation
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Gene Expression
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Trans-Activators
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Up-Regulation
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Cells, Cultured
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Sensitivity and Specificity
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Protein Serine-Threonine Kinases
Type of study:
Diagnostic_studies
Limits:
Humans
Language:
En
Journal:
Chinese Journal of Traumatology
Year:
2002
Type:
Article