Molecular biological survey of tick-born arboviruses in southern part of Xinjiang / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 325-330, 2005.
Article
in Chinese
| WPRIM
| ID: wpr-333013
ABSTRACT
<p><b>BACKGROUND</b>To disclose the species and distribution of tick-borne arboviruses in the southern part of Xinjiang.</p><p><b>METHOD</b>Totally 5045 ticks were collected from 36 collecting sites of 23 places in the southern Xinjiang, which were made into cDNA pools with pd(N)6 primer through RT-PCR method. Then PCR was used to detect viral nucleotide sequence from cDNA.</p><p><b>RESULTS</b>All 34 cDNAs showed negative to flavivirus and California serogroup virus primers; but nairovirus and primers derived from Xinjiang hemorrhagic fever virus had amplified and yielded some obvious bands corresponding to the nucleotide sequences of Xinjiang hemorrhagic fever virus. A phylogenetic analysis was done to the obtained partial sequences of L and S segments.</p><p><b>CONCLUSION</b>Nucleotide sequences of Neither flaviviruses nor California serogroup viruses were detected from the samples. However partial L segment sequence was first reported in China. Phylogenetic analysis of partial L and S segments disclosed the molecular characteristic of Xinjiang hemorrhagic fever virus.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Phylogeny
/
Arboviruses
/
Ticks
/
Virology
/
China
/
Classification
/
Hemorrhagic Fever Virus, Crimean-Congo
/
Tick-Borne Diseases
/
Sequence Analysis, DNA
/
Reverse Transcriptase Polymerase Chain Reaction
Limits:
Animals
Country/Region as subject:
Asia
Language:
Chinese
Journal:
Chinese Journal of Experimental and Clinical Virology
Year:
2005
Type:
Article
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