Molecular characteristics of three new isolates of Japanese encephalitis virus in Fujian Province / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
; (6): 5-8, 2005.
Article
in Zh
| WPRIM
| ID: wpr-333065
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To analyze the genotype of newly isolated Japanese encephalitis viruses in Fujian province and the characteristics of amino acid sequence in the E gene.</p><p><b>METHODS</b>PrM and E segments of newly isolated Japanese encephalitis viruses were amplified by RT-PCR, the PCR products were cloned into T vector for sequencing. Phylogenetic analysis was carried out by PHYLIP program.</p><p><b>RESULTS</b>Newly isolated Japanese encephalitis viruses belonged to genotype III, the nucleotide and amino acid of E gene showed high homology to vaccine strain SA14-14-2, but had some difference in some domains.</p><p><b>CONCLUSION</b>Newly isolated viruses in Fujian province belonged to Japanese encephalitis virus genotype III. E protein of the isolates showed some differences as compared with vaccine strains.</p>
Full text:
1
Index:
WPRIM
Main subject:
Phylogeny
/
Virology
/
RNA, Viral
/
Molecular Sequence Data
/
Chemistry
/
China
/
Viral Envelope Proteins
/
Amino Acid Sequence
/
Classification
/
Genome, Viral
Limits:
Animals
/
Humans
Country/Region as subject:
Asia
Language:
Zh
Journal:
Chinese Journal of Experimental and Clinical Virology
Year:
2005
Type:
Article