Molecular characteristics of three new isolates of Japanese encephalitis virus in Fujian Province / 中华实验和临床病毒学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To analyze the genotype of newly isolated Japanese encephalitis viruses in Fujian province and the characteristics of amino acid sequence in the E gene.</p><p><b>METHODS</b>PrM and E segments of newly isolated Japanese encephalitis viruses were amplified by RT-PCR, the PCR products were cloned into T vector for sequencing. Phylogenetic analysis was carried out by PHYLIP program.</p><p><b>RESULTS</b>Newly isolated Japanese encephalitis viruses belonged to genotype III, the nucleotide and amino acid of E gene showed high homology to vaccine strain SA14-14-2, but had some difference in some domains.</p><p><b>CONCLUSION</b>Newly isolated viruses in Fujian province belonged to Japanese encephalitis virus genotype III. E protein of the isolates showed some differences as compared with vaccine strains.</p>