Adenovirus containing c-FLIPs gene induces antiapoptosis activation of lymphocyte in vitro / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 114-117, 2005.
Article
in Chinese
| WPRIM
| ID: wpr-333087
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the efficacy and mechanisms of recombinant adenovirus containing c-FLIPs (cellular FLICE-inhibitory protein short) in inducing antiapoptosis activation of lymphocyte in vitro.</p><p><b>METHODS</b>c-FLIPs gene was cloned from total RNA of lymphocyte with RT-PCR methods, then constructed recombined adenovirus Ad.c-FLIPs by pAdeasy system. H9 cells infected with Ad.c-FLIPs produced antiapoptosis derived from anti-Apo-1 antibody, and those antiapoptosis evaluated with PI stain flow cytometry (FCM) and Hoechst stain.</p><p><b>RESULTS</b>c-FLIPs gene was successfully cloned from lymphocytes and incorporated into recombinant adenovirus Ad.c-FLIPs.After exposure to anti-Apo-1 for 24 h, the apoptosis of H9 cells preconditioned by Ad.c-FLIPs for 24 h as measured by Hochst stain or FCM significantly decreased, compared with non-preconditioned cells, 3.60% +/- 0.21% vs. 48.33% +/- 7.41%, respectively, indicating that Ad.c-FLIPs preconditioning protected H9 cells against apoptosis induced by anti-Apo-1.</p><p><b>CONCLUSION</b>Recombinant adenovirus Ad.c-FLIPs effectively induced anti-apoptosis activation of lymphocyte.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Physiology
/
Lymphocytes
/
Transfection
/
CD4-Positive T-Lymphocytes
/
Cell Line
/
Adenoviridae
/
Apoptosis
/
Fas Receptor
/
Reverse Transcriptase Polymerase Chain Reaction
/
Cell Biology
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Experimental and Clinical Virology
Year:
2005
Type:
Article
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