ISGF3, a critical factor of the IFN-alpha pathway in the antiviral action of HBV / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
; (6): 110-113, 2005.
Article
in Zh
| WPRIM
| ID: wpr-333088
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the mechanism of signal transduction in anti-HBV action of IFN-alpha.</p><p><b>METHODS</b>The HBV DNA in HepG 2.2.15 cell line supernatant with/without IFNalpha-2b were monitored by fluorescence real-time quantitive PCR. STAT1, STAT2, ISGF3-gamma, PKR, 2'5'-OAS mRNA levels from HepG 2 and HepG 2.2.15 cell lines that were treated with/without IFNalpha-2b at different times were detected by semi-quantitive RT-PCR. And the ISGF3-gamma protein was detected by Western blot. Then, these items were detected again after inhibiting the JAK-STAT pathway with genistein.</p><p><b>RESULTS</b>The HBV DNA in 2215 supernatant that were treated with IFNalpha-2b for 8 hours decreased 0.72 log 10 copies/ml. But the basal levels of DNA in cells pretreated with genistein? followed by IFNalpha-2b did not decrease. The STAT1, STAT2, ISGF3-gamma, 2'5'-OAS, PKR mRNA levels were upregulated by IFNalpha-2b. The same phenomena were observed with STAT1, STAT2, ISGF3-gamma mRNA when pretreated with genistein then treated with IFNalpha-2b, but the levels of 2'5'-OAS, PKR mRNA were decreased in this situation. The expression of the protein of ISGF3-gamma was also augmented by IFNalpha-2b, and was blocked by genistein.</p><p><b>CONCLUSION</b>The JAK-STAT pathway seems to be a critical pathway in IFNalpha-2b action against HBV? and ISGF3 is most probably a key factor of the route.</p>
Full text:
1
Index:
WPRIM
Main subject:
Antiviral Agents
/
Pharmacology
/
DNA, Viral
/
RNA, Messenger
/
Signal Transduction
/
Gene Expression Regulation, Neoplastic
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Hepatitis B virus
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Blotting, Western
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Polymerase Chain Reaction
/
Interferon-alpha
Type of study:
Guideline
Limits:
Humans
Language:
Zh
Journal:
Chinese Journal of Experimental and Clinical Virology
Year:
2005
Type:
Article