Acquired somatic mutations in non-coding region of bcl-6 gene in germinal center-type diffuse large B-cell lymphoma / 中华病理学杂志
Chinese Journal of Pathology
; (12): 595-599, 2010.
Article
in Zh
| WPRIM
| ID: wpr-333202
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the mutation of 5' non-coding region of bcl-6 gene in germinal center B-cell (GCB) subtype of diffuse large B-cell lymphoma (DLBCL).</p><p><b>METHODS</b>t(14;18) detection and immunohistochemical staining (EnVision method) were performed in 60 cases of DLBCL, which were divided into GCB and non-GCB subtypes. Polymerase chain reaction (PCR), single-strand conformation polymorphism and direct DNA sequencing were used to identify mutations in the 5' non-coding region of the bcl-6 gene.</p><p><b>RESULTS</b>Seven of 60 cases showed t(14;18) translocation in the major breakpoint region. Using minimally acceptable criteria, 18 of 60 cases were probably to be germinal centre derived. Bcl-6 mutations were detected in 12 of 60 cases (20.0%) of DLBCL, with a significantly higher frequency in the GCB subgroups (7/18) than in the non-GCB subgroups (11.9%, 5/42). Bcl-6 mutations occurred most frequently in +363 and +469 sites. An association of bcl-6 mutation and GCB subgroup was obtained.</p><p><b>CONCLUSIONS</b>The 5' regulatory region of the bcl-6 gene underwent less frequent somatic hypermutation during lymphomagenesis than the results of previous reports. Bcl-6 mutation occurred mostly in the GCB subtype and detection of t(14;18) seems helpful in the classification of DLBCL.</p>
Full text:
1
Index:
WPRIM
Main subject:
Pathology
/
Translocation, Genetic
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Chromosomes, Human, Pair 14
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Chromosomes, Human, Pair 18
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DNA Mutational Analysis
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B-Lymphocytes
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Lymphoma, Large B-Cell, Diffuse
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Point Mutation
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Germinal Center
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DNA-Binding Proteins
Type of study:
Prognostic_studies
Limits:
Adult
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Aged
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Aged80
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Female
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Humans
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Male
Language:
Zh
Journal:
Chinese Journal of Pathology
Year:
2010
Type:
Article