Effect of cyclic-tension force on the expression of osteogenesis genes in human periodontal ligament cells / 中华口腔医学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the effect of cyclic-tension force on the expression of osteogenesis genes in human periodontal ligament cells (HPDLC).</p><p><b>METHODS</b>HPDLC were cultured on flexible-bottomed plates and subjected to 12% elongation by strain unit at 6 cycles/min (i.e.5-s elongation and 5-s relaxation) for 48 hours in the experimental groups. GEArray Q series Human Osteogenesis Gene Array was used to identify the genes expressed in HPDLC, including growth factors and associated molecules, extracellular matrix and its associated proteins, cell adhesion molecules and housekeeping genes. The changes in the expression of 96 representative transcripts were determined by arrayed cDNA hybridization.</p><p><b>RESULTS</b>After application of tension force, 21 genes were significantly upregulated, including 10 growth factors and associated molecules, 10 extracellular matrix and its associated protein and 1 cell adhesion molecules. Two genes were significantly downregulated, including 1 growth factors and associated molecules and 1 cell adhesion molecules.</p><p><b>CONCLUSIONS</b>The HPDLC can differentiate into osteoblast-like cells by mechanical stretch induction through the activation of some osteogenesis genes.</p>