Construction of antisense human tankyrase-1 RNA retroviral vector and its inhibition on tongue cancer cells / 中华口腔医学杂志
Chinese Journal of Stomatology
;
(12): 180-183, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-333373
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the inhibition of telomerase activity and cellular proliferation in tongue cancer TCCA-8113 cell lines by antisense human tankyrase-1 RNA treatment, and explore the possibility of the tankyrase-1 as a target of gene therapy for tongue cancer.</p><p><b>METHODS</b>The replication deficient retrovirus expressing tankyrase-1 antisense RNA was constructed to infect the TCCA-8113 cells. Tankyrase-1 expression was examined by RT-PCR. Telomerase activity was assayed by telomerase repeat amplification protocol (TRAP). Cell proliferation was investigated by cellular growth curve. Cellular apoptosis was detected by flow cytometry method and invert microscope.</p><p><b>RESULTS</b>Tankyrase-1 expression and telomerase activity of tongue cancer TCCA-8113 cells were significantly inhibited. There was G(1)-S phase arrest when TCCA-8113 cells were treated with antisense tankyrase-1 transduction. Cellular proliferation was arrested, and cellular apoptosis occurred after antisense tankyrase transduction.</p><p><b>CONCLUSIONS</b>The transduction of antisense tankyrase-1 by retroviral vector can significantly inhibit the tankyrase-1 expression and telomerase activity of tongue cancer TCCA-8113 cell lines, and arrest the cellular proliferation and promote cellular apoptosis. The tankyrase may be a potential target of gene therapy for tongue cancer.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Retroviridae
/
Therapeutics
/
Tongue Neoplasms
/
Transfection
/
Genetic Therapy
/
RNA, Antisense
/
Apoptosis
/
Telomerase
/
Tankyrases
Type of study:
Practice guideline
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Stomatology
Year:
2007
Type:
Article
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