Increased expression of serum gelsolin in patients with osteosarcoma / 中华医学杂志(英文版)
Chinese Medical Journal
;
(24): 262-269, 2012.
Article
in English
| WPRIM
| ID: wpr-333504
ABSTRACT
<p><b>BACKGROUND</b>Identification of potential serum biomarkers of osteosarcoma to aid in its early diagnosis and in the discovery of possible therapeutic targets is an area of increasing interest.</p><p><b>METHODS</b>Two-dimensional difference-in-gel electrophoresis was used to assess multiple serum samples in patients with osteosarcoma. In addition, differential expression of protein biomarkers was characterized in osteosarcoma serum by using matrix-assisted desorption/ionization time-of-flight mass spectrometry coupled with database interrogation. Serum samples from four individuals with osteosarcoma and four age- and sex-matched healthy control subjects were compared.</p><p><b>RESULTS</b>Fifty-eight significant protein spot features in the osteosarcoma sera were found. These spot features were excised, digested with trypsin, and analyzed with mass spectrometry. Gelsolin was down-regulated only in osteosarcoma. Furthermore, Western blotting and enzyme linked immunosorbent assay (ELISA) confirmed decreased levels of gelsolin in the osteosarcoma serum samples.</p><p><b>CONCLUSIONS</b>These results indicated that gelsolin might have great potential as a biomarker of osteosarcoma and as a potential target for gene therapy.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Blood
/
Enzyme-Linked Immunosorbent Assay
/
Electrophoresis, Gel, Two-Dimensional
/
Biomarkers, Tumor
/
Osteosarcoma
/
Blotting, Western
/
Gelsolin
/
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Type of study:
Screening study
Limits:
Adolescent
/
Adult
/
Child
/
Female
/
Humans
/
Male
Language:
English
Journal:
Chinese Medical Journal
Year:
2012
Type:
Article
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