Effect of DNase I on biofilm formation of Staphylococcus aureus / 南方医科大学学报

ABSTRACT

<p><b>OBJECTIVE</b>To study the effect of DNase I on biofilm formation of Staphylococcus aureus.</p><p><b>METHODS</b>The growth curve of S. aureus was detected using a spectrophotometer. The adhesion of S. aureus was analyzed using flat colony counting method, and the biofilm formation was assayed using the 96-well crystal violet staining method.</p><p><b>RESULTS</b>Exposure to different concentrations of DNase I did not obviously affect the growth of S. aureus but significantly inhibit the formation of bacterial biofilms in a dose-dependent manner. DNase I inhibited the adhesion of S. aureus at different growth stages. When combined with antibiotics, DNase I resulted in a signi?cant decrease in the established bio?lm biomass compared to antibiotics or DNase I used alone.</p><p><b>CONCLUSION</b>DNase I can effectively inhibit biofilm formation of S. aureus and enhance the inhibitory effect of antibiotics against S. aureus biofilms.</p>