Minocycline reduces hyperpolarization-activated current in rat substantia gelatinosa neurons / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1155-1161, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-333665
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of minocycline on hyperpolarization-activated current (Ih) in the substantia gelatinosa (SG) neurons in rat spinal dorsal horn.</p><p><b>METHODS</b>In vitro spinal cord transverse slices were prepared from 3-5-week-old male Sprague-Dawley rats. Using whole-cell patch clamp technique, Ih currents were recorded before and after bath application of minocycline (1-300 µmol/L) to the SG neurons.</p><p><b>RESULTS</b>Ih currents were observed in nearly 50% of the recorded neurons, and were blocked by Ih blocker CsCl and ZD7288. Minocycline rapidly and reversibly reduced the amplitude of Ih and decreased the current density in a concentration-dependent manner with an IC50 of 34 µmol/L.</p><p><b>CONCLUSION</b>Minocycline suppresses the excitability of SG neurons through inhibiting the amplitude and current density of Ih and thereby contributes to pain modulation.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Substantia Gelatinosa
/
Rats, Sprague-Dawley
/
Patch-Clamp Techniques
/
Cell Biology
/
Minocycline
/
Neurons
Limits:
Animals
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2015
Type:
Article
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