Influence of vasoactive intestinal peptide on expression of pulmonary surfactant associated protein A in lung explants / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 117-120, 2004.
Article
in Chinese
| WPRIM
| ID: wpr-333699
ABSTRACT
<p><b>AIM</b>To study the influence of VIP on the expression of SP-A and its intracellular signal transduction pathway.</p><p><b>METHODS</b>The influence of VIP on the expression of SP-A was studied by immunohistochemistry and RT-PCR. The intracellular signal transduction pathway was further investigated by using receptor antagonist, protein kinase inhibitor and antisense oligonucleotides.</p><p><b>RESULTS</b>(1) VIP(10(-8) mol/L) enhanced SP-A protein expression in alveolar type II cells (ATII) and increased the content of SP-A mRNA in lung tissue. (2) VIP receptor antagonist [D-P-C1-Phe (6)-Leu (17)]-VIP (10(-6) mol/L) could suppress the VIP-induced expression of SP-A protein and SP-A mRNA. (3) c-fos antisense oligonucleotides (9 x 10(-6) mol/L) could inhibit the VIP-induced expression of SP-A protein and SP-A mRNA. (4) Protein kinase C(PKC) inhibitor H7 (10(-5) mol/L) could also depress the V1P-induced SP-A protein and SP-A mRNA.</p><p><b>CONCLUSION</b>VIP can up-regulate the expression of SP-A through its receptor. PKC and c-fos protein play important roles in the intracellular signal transduction pathway through which VIP induces the expression of SP-A.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Pulmonary Alveoli
/
In Vitro Techniques
/
Protein Kinase C
/
Vasoactive Intestinal Peptide
/
Signal Transduction
/
Proto-Oncogene Proteins c-fos
/
Rats, Wistar
/
Cell Biology
/
Pulmonary Surfactant-Associated Protein A
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Applied Physiology
Year:
2004
Type:
Article
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