The effect of LPC on the pacemaker current I(f) in ischemic myocardium and the influence of ISO on it / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 1-5, 2004.
Article
in Chinese
| WPRIM
| ID: wpr-333735
ABSTRACT
<p><b>AIM</b>To observe the effect of LPC on the pacemaker current I(f) in ischemic myocardium and if the effect could be reversed by ISO.</p><p><b>METHODS</b>By using two microelectrode voltage clamp technique to measure and compare the amplitude of I(f) of ischemic myocardium in the presence of LPC and LPC add ISO.</p><p><b>RESULTS</b>Ischemia decreased the amplitude of I(f) at all membrane potential levels. Adding LPC 2 x 10(-5) mol/L to the ischemia-like solution, the amplitude of I(f) decreased further (n = 5, P < 0.05), it means that LPC aggravated the inhibitory effect of "ischemia" on the pacemaker activity. Adding LPC 2 x 10(-5) mol/L and ISO 1 x 10(-6) mol/L together to the ischemia-like solution, the amplitude of I(f) increased significantly at membrane potential -90 mV to - 120 mV (n = 8, P < 0.05) compared with ischemia condition, but still did not reach the levels before ischemia.</p><p><b>CONCLUSION</b>In acute myocardial ischemia condition, toxic metabolite LPC accentuated its inhibitory effect on pacemaker current I(f), a local release and accumulation of catecholamine could not completely reverse their inhibitory effect.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Sheep
/
Lysophosphatidylcholines
/
Myocardial Ischemia
/
Patch-Clamp Techniques
/
Isoproterenol
/
Membrane Potentials
/
Metabolism
/
Microelectrodes
/
Myocardium
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Applied Physiology
Year:
2004
Type:
Article
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