Effects of oxygen-glucose deprivation on cultured rat hippocampal neurons / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 313-317, 2003.
Article
in Chinese
| WPRIM
| ID: wpr-333766
ABSTRACT
<p><b>AIM</b>To investigate the effects of oxygen-glucose deprivation on cultured rat hippocampal neurons.</p><p><b>METHODS</b>The hippocampal neurons cultured for 12 d were exposed to combined oxygen-glucose deprivation for 0.5 - 4 h and then cultured with original medium in normoxia for 28 h. Necrotic neurons were identified by 0.4% trypan blue staining and apoptotic neurons were detected by a TUNEL technique. Meanwhile, the area, perimeter and circle diameter of cell bodies were measured respectively by a photography analysis system.</p><p><b>RESULTS</b>The percentage of necrotic cells in cultured hippocampal neurons increased significantly during oxygen-glucose deprivation, but the percentage of apoptotic cells increased significantly after 28 h oxygen-glucose recovery. Photography analysis showed that area, perimeter and circle diameter of the necrotic cell bodies were larger than those of the apoptotic ones.</p><p><b>CONCLUSION</b>Oxygen-glucose deprivation can lead to severe damage of cultured hippocampal neurons. The necrosis is major during acute oxygen-glucose deprivation, while the apoptosis is major 28 h after oxygen-glucose recovery.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Oxygen
/
Cell Hypoxia
/
Cells, Cultured
/
Rats, Wistar
/
Cell Biology
/
Glucose
/
Hippocampus
/
Metabolism
/
Neurons
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Applied Physiology
Year:
2003
Type:
Article
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