Glucocorticoids up-regulate human chorion 11beta-hydroxysteroid dehydrogenase type 1 / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 291-294, 2003.
Article
in Chinese
| WPRIM
| ID: wpr-333772
ABSTRACT
<p><b>AIM</b>To study the co-localization of glucocorticoid receptor and 11beta-hydroxysteroid dehydrogenase 1 (11beta-HSD1) and to investigate whether glucocorticoids regulate the reductase activity and expression of 11beta-HSD1.</p><p><b>METHODS</b>Immunohistochemical staining for 11beta-HSD1 in cultured primary human chorionic trophoblasts was performed. Radiometric conversion assay and Northern blot analysis were respectively used to observe the activity and mRNA expression of 11beta-HSD1.</p><p><b>RESULTS</b>11beta-HSD1 and GR were co-expressed in the same chorionic trophoblast. Both 11beta-HSD1 reductase activity and mRNA levels were increased by dexamethasone (10(-6) mol/L, 10(-7) mol/L) in the cultured chorionic trophoblasts, and the effects were blocked by GR antagonist RU486 (10(-6) mol/L).</p><p><b>CONCLUSION</b>By binding to GR, glucocorticoids induce the expression of 11beta-HSD1 by a possible intracrine mechanism.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
RNA, Messenger
/
Up-Regulation
/
Cells, Cultured
/
Chorion
/
11-beta-Hydroxysteroid Dehydrogenase Type 1
/
Genetics
/
Glucocorticoids
/
Metabolism
Limits:
Female
/
Humans
Language:
Chinese
Journal:
Chinese Journal of Applied Physiology
Year:
2003
Type:
Article
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