Establishment of transgenic mice with visualized neovascularization / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1748-1752, 2011.
Article
in Chinese
| WPRIM
| ID: wpr-333822
ABSTRACT
<p><b>OBJECTIVE</b>To establish transgenic mice with GFP expression in the vascular endothelium during neovascularization.</p><p><b>METHODS</b>The vector nestin-hsp68-gfp containing nestin second intron was introduced into U251 cells and the expression level of GFP was detected by fluorescence microscopy. Transgenic mice were produced by microinjection. The genome of the offspring mice was screened by PCR, and GFP expression in the vascular endothelium was detected using immunohistochemistry.</p><p><b>RESULTS</b>Thirteen offspring mice were obtained and 2 of them were positive for GFP in the vascular endothelium as detected by PCR. GFP was detected in the offspring mice both at the embryonic stage and after birth.</p><p><b>CONCLUSIONS</b>The transgenic mice with GFP expression in the vascular endothelium during neovascularization have been successfully established.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Endothelium, Vascular
/
Mice, Transgenic
/
Molecular Sequence Data
/
Base Sequence
/
Neovascularization, Physiologic
/
Green Fluorescent Proteins
/
Nestin
/
Genetics
/
Intermediate Filament Proteins
/
Animals, Newborn
Limits:
Animals
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2011
Type:
Article
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