Development of a time-resolved fluoroimmunoassay for detecting S1 subunit of pertussis toxin and its application / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1509-1512, 2011.
Article
in Chinese
| WPRIM
| ID: wpr-333876
ABSTRACT
<p><b>OBJECTIVE</b>To develop a time-resolved fluoroimmunoassay (TRFIA) for detection of pertussis toxin (PT) S1 subunit for quality control of human PT vaccine.</p><p><b>METHODS</b>A double antibody sandwich one-step method was used to establish the TRFIA for detecting PT S1 subunit in the vaccine.</p><p><b>RESULTS</b>The sensitivity of c peptide analysis reached 2.5 ng/ml without cross-reactions with other antigens. This assay could be used in detecting S1 subunit in the vaccine.</p><p><b>CONCLUSION</b>The TRFIA for detecting PT S1 subunit is simple, sensitive and rapid for quality control of the PT vaccine.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Quality Control
/
Reference Standards
/
Pertussis Vaccine
/
Fluoroimmunoassay
/
Chemistry
/
Sensitivity and Specificity
/
Cross Reactions
/
Pertussis Toxin
/
Methods
Type of study:
Diagnostic study
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2011
Type:
Article
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