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Stem cell characteristics and islet differentiation potential of human amniotic epithelial cells / 南方医科大学学报
Article in Zh | WPRIM | ID: wpr-333881
Responsible library: WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To establish a method for isolating and culturing human amniotic epithelial cells (hAECs) in a serum-free medium and investigate their transdifferentiation ability into islet-like cells.</p><p><b>METHODS</b>The culture condition of hAECs was optimized using DMEM with different supplements. The genetic stability of the tenth-passage cells was assessed by chromosome analysis and G-banding method. The stem cell characteristics of the cells were identified by examination of the surface markers using immunofluorescence methods. The endocrine-related genes and hormones of the cells were tested after induced differentiation into islet-like cells.</p><p><b>RESULTS</b>The hAECs allow stable passaging in the presence of 10 ng epidermal growth factor (EGF) in the culture medium. After 10 passages, the cells maintained a normal karyotype and G-banding profile. The hAECs expressed many multi-potent stem cell markers, including SSEA4, TRA-1-60, and TRA-1-81. After induced differentiation, the endocrine-related genes were expressed in the islet-like cells, including PDX1, ngn3, insulin and glucagon. Insulin secretion increased in the differentiated islet-like cells in response to high glucose exposure.</p><p><b>CONCLUSION</b>We established a method for isolating and expanding the hAECs in a serum-free medium. hAECs possess stem cell characteristics and can be induced to differentiate into islet-like cells in vitro.</p>
Subject(s)
Full text: 1 Index: WPRIM Main subject: Stem Cells / Cell Differentiation / Cells, Cultured / Islets of Langerhans / Cell Culture Techniques / Bodily Secretions / Cell Biology / Epithelial Cells / Amnion / Insulin Type of study: Prognostic_studies Limits: Humans Language: Zh Journal: Journal of Southern Medical University Year: 2011 Type: Article
Full text: 1 Index: WPRIM Main subject: Stem Cells / Cell Differentiation / Cells, Cultured / Islets of Langerhans / Cell Culture Techniques / Bodily Secretions / Cell Biology / Epithelial Cells / Amnion / Insulin Type of study: Prognostic_studies Limits: Humans Language: Zh Journal: Journal of Southern Medical University Year: 2011 Type: Article