Influence of beta-amyloid protein and cholesterol on the pathological changes of Alzheimer's disease and expression of nicotinic acetylcholine receptors in rats / 中华病理学杂志
Chinese Journal of Pathology
;
(12): 184-189, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-333935
ABSTRACT
<p><b>OBJECTIVE</b>To study the influence of beta-amyloid protein (Abeta) and cholesterol on the pathological changes of Alzheimer's disease (AD) and on the expression of nicotinic acetylcholine receptor (nAChR) subunits in the brains of rats.</p><p><b>METHOD</b>The rats were treated by intracerebroventricular injection of Abeta1-42 and fed with a diet containing 5% cholesterol to establish animal model of AD. The pathological changes, learning and memory, and expression of nAChRs of rats were analyzed by Bieoschowsky staining, immunohistochemistry, water-labyrinth, Western blot, and RT-PCR.</p><p><b>RESULTS</b>Abeta intracerebroventricular injection induced Abeta deposition in rat brains and high-cholesterol diet resulted in hypercholesterolemia in the animals. Injection of Abeta caused a reduction of learning and memory of rats and modifications of the expression of nAChRs. Cholesterol enhanced these effects of Abeta on neuropathology and expression of nAChRs.</p><p><b>CONCLUSIONS</b>Abeta can induce marked neuropathological changes, influence the learning and study ability, and modify the expression of nAChRs. Cholesterol can enhance the neurotoxicity of Abeta.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Peptide Fragments
/
Blood
/
RNA, Messenger
/
Random Allocation
/
Cerebral Cortex
/
Cholesterol
/
Amyloid beta-Peptides
/
Receptors, Nicotinic
/
Rats, Wistar
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Pathology
Year:
2007
Type:
Article
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