Detection of Mycobacterium tuberculosis DNA in sarcoidosis samples using real-time fluorescence polymerase chain reaction / 中华病理学杂志
Chinese Journal of Pathology
;
(12): 735-737, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-333982
ABSTRACT
<p><b>OBJECTIVE</b>To study the role of Mycobacterium tuberculosis in the pathogenesis of sarcoidosis.</p><p><b>METHODS</b>Archival material of 22 patients with a histologic diagnosis of sarcoidosis were retrieved. Real-time fluorescent polymerase chain reaction (PCR) was used to detect DNA fragments of the complex-specific insertion sequence IS6110 of Mycobacterium tuberculosis in formalin-fixed and paraffin-embedded biopsy samples.</p><p><b>RESULTS</b>Among the 22 samples studied, Mycobacterium tuberculosis DNA was detected in 11 cases. The sequence of PCR amplified IS6110 DNA fragments completely matched with the related sequence in Mycobacterium tuberculosis gene.</p><p><b>CONCLUSIONS</b>Mycobacterium tuberculosis DNA is identified in a certain proportion of patients with a clinicopathologic diagnosis of sarcoidosis. Mycobacterium tuberculosis may be an important etiologic agent, at least in some of these patients.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Sarcoidosis
/
DNA, Bacterial
/
Polymerase Chain Reaction
/
Follow-Up Studies
/
Paraffin Embedding
/
Fluorescence
/
Genetics
/
Lymph Nodes
/
Methods
Type of study:
Diagnostic study
/
Observational study
/
Prognostic study
Limits:
Adult
/
Female
/
Humans
/
Male
Language:
Chinese
Journal:
Chinese Journal of Pathology
Year:
2006
Type:
Article
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