Expression of P27(kip1) and cyclin G in patients with acute leukemia and its correlation / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 847-851, 2009.
Article
in Zh
| WPRIM
| ID: wpr-334011
Responsible library:
WPRO
ABSTRACT
This study was purposed to explore the expression of P27(kip1)and cyclin G in patients with acute leukemia (AL) and its correlation. The reverse polymerase chain reaction (RT-PCR) was used to analyse the expression of P27(kip1) and cyclin G mRNA in 89 AL patients and 10 normal persons; Western blot was used to analyze the expression of P27(kip1) and cyclin G protein in 39 AL patients and 10 normal persons. The results showed that the cyclin G mRNA and protein expressions in new diagnosed/relapsed cases of AL were significantly higher than those in patients with remission and normal controls (p < 0.05 and p < 0.01), but there was no difference between remission cases and normal controls (p > 0.05). The expression of P27(kip1) mRNA in newly diagnosed/relapsed patients with AL was not significantly different from patients with remission and normal controls (p > 0.05), while the P27(kip1) protein expression in remission cases of AL and normal controls was significantly higher than that in new diagnosed/relapsed cases (p < 0.05), but there was no difference between remission cases and normal controls (p > 0.05). The expression of P27(kip1) negatively and lowly correlated with the expression of cyclin G in patients with AL. It is concluded that the low expression of P27(kip1) and the high expression of cyclin G in patients with AL may have some correlation with genesis and development of AL and may be an indication for poor prognosis of AL.
Full text:
1
Index:
WPRIM
Main subject:
Leukemia, Myeloid, Acute
/
Cyclin-Dependent Kinase Inhibitor p27
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Cyclin G1
/
Genetics
/
Metabolism
Limits:
Adult
/
Female
/
Humans
/
Male
Language:
Zh
Journal:
Journal of Experimental Hematology
Year:
2009
Type:
Article