Analysis of variant translocation der ins (17; 15) in patient with APL by G-banding technique and interphase fluorescence in situ hybridization / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 537-540, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-334074
ABSTRACT
To investigate the biological characteristics of the variant translocation der ins (17;15) in a patient with acute promyelocytic leukemia (APL), the conventional G-banding technique, interphase fluorescence in situ hybridization (int-FISH), RT-PCR, gene scanning, gene sequence and flow cytometry were performed. The results indicated that the variant translocation der ins (17, 15) observed by G banding technique was a rare type, the int-FISH assay by using dual-color pml/raralpha fusion probes confirmed the cytogenetic findings. The detection results of other molecular methods demonstrated the existence of the whole pml/raralpha fusion gene, while this case had insertion variant translocation. This patient got complete remission by using combined chemotherapy, and survives with continuous complete remission during following up for 1 year. In conclusion, the variant translocation der ins (17; 15) is rare type in APL, its incidence is lower, several signal types in detection of int-FISH were observed and the combination chemotherapy for this patient showed more obvious efficacy.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Translocation, Genetic
/
Chromosomes, Human, Pair 15
/
Chromosomes, Human, Pair 17
/
Leukemia, Promyelocytic, Acute
/
Chromosome Banding
/
In Situ Hybridization, Fluorescence
/
Genetics
/
Interphase
/
Methods
Limits:
Humans
/
Male
Language:
Chinese
Journal:
Journal of Experimental Hematology
Year:
2009
Type:
Article
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