Identification of up-regulated genes induced by angiotensin II in cardiac fibroblasts / 生理学报
Acta Physiologica Sinica
;
(6): 643-647, 2005.
Article
in Chinese
| WPRIM
| ID: wpr-334122
ABSTRACT
To identify up-regulated genes in adult rat cardiac fibroblasts (CF) induced by angiotensin II (Ang II), suppression subtractive hybridization (SSH) was performed between the CF stimulated by Ang II (tester) and unstimulated CF (driver) to generate subtractive cDNA library. The library was screened with dot blots hybridization to further verify the differentially expressed cDNA clones. Partial positive clones (19 up-regulated genes) were sequenced and BLAST analyzed. Twelve up-regulated genes related to extracellular matrix, cell cycle, intracellular signal transduction, cell cytoskeleton, cell metabolism and 7 new expressed sequence tags (EST) were acquired (GenBank accession number CN382808, CN382809, CN382810, CN382811, CN382812, CN382813, CN382814). Our data reveal that SSH is a powerful technique of high sensitivity for the detection and cloning of up-regulated genes expressed in CF induced by Ang II, which may be helpful to clarify the mechanism of cardiac remodeling.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Angiotensin II
/
Up-Regulation
/
Cells, Cultured
/
Gene Expression Regulation
/
Rats, Sprague-Dawley
/
DNA, Complementary
/
Expressed Sequence Tags
/
Ventricular Remodeling
/
Cell Biology
Type of study:
Diagnostic study
Limits:
Animals
Language:
Chinese
Journal:
Acta Physiologica Sinica
Year:
2005
Type:
Article
Similar
MEDLINE
...
LILACS
LIS