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Promoting effects of serum-free murine bone marrow endothelial cell conditioned medium on the growth of bone marrow endothelial cells / 生理学报
Acta Physiologica Sinica ; (6): 199-204, 2005.
Article in Chinese | WPRIM | ID: wpr-334186
ABSTRACT
To study the effects of serum-free murine bone marrow endothelial cell conditioned medium (mBMEC-CM) on the growth of bone marrow endothelial cells, mBMEC-CM was collected and ultrafiltrated by Centriprep-10. The retentate of mBMEC-CM [molecular weight (MW)>10 kDa] and the filtrate of mBMEC-CM (MW<10 kDa) were obtained. The effect of bone marrow conditioned media, their components and exogenous cytokines on the formation of endothelial cell colonies were observed. The effect of bone marrow conditioned media, their components and exogenous cytokines on the proliferation of murine bone marrow endothelial cells were determined by [(3)H]-thymidine incorporation. The method of hybridizing to the Atlas cDNA array was used to determine the expression of cytokine mRNAs in bone marrow endothelial cells. The results obtained are as follows vWF was expressed in bone marrow endothelial cells. The original mBMEC-CM and MW>10 kDa component of mBMEC-CM promoted the proliferation of bone marrow endothelial cell colonies and increased [(3)H]-thymidine incorporation of bone marrow endothelial cells. The MW<10 kDa component did not affect the production of endothelial cell colonies and did not increase [(3)H]-thymidine incorporation of endothelial cells. Six cytokines (IL-6, IL-11, SCF, GM-CSF, VEGF, bFGF) promoted the proliferation of bone marrow endothelial cell colonies. VEGF, bFGF and SCF increased [(3)H]-thymidine incorporation of bone marrow endothelial cells. According to the results of the Atlas cDNA array, GM-CSF,TGF-beta,BMP-2, bFGF, SCF, endothelin-2, thymosin beta10, MSP-1, connective tissue GF, PDGF-A chain, MIP-2 alpha, PlGF, neutrophil activating protein ENA-78, INF-gamma, IL-1, IL-6, IL-13, IL-11, inhibin-alpha mRNAs were expressed in endothelial cells. These results suggest that murine bone marrow endothelial cell conditioned medium promotes the proliferation of bone marrow endothelial cells.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Pharmacology / Physiology / Bone Marrow Cells / Cell Line / Cells, Cultured / Culture Media, Serum-Free / Culture Media, Conditioned / Cell Biology / Endothelial Cells / Cell Proliferation Limits: Animals Language: Chinese Journal: Acta Physiologica Sinica Year: 2005 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pharmacology / Physiology / Bone Marrow Cells / Cell Line / Cells, Cultured / Culture Media, Serum-Free / Culture Media, Conditioned / Cell Biology / Endothelial Cells / Cell Proliferation Limits: Animals Language: Chinese Journal: Acta Physiologica Sinica Year: 2005 Type: Article