Effect of RAR-beta transfection on the proliferation and phenotype of rat hepatic stellate cells / 中华肝脏病杂志
Chinese Journal of Hepatology
;
(12): 297-300, 2002.
Article
in Chinese
| WPRIM
| ID: wpr-334220
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect of RAR-beta transfection plus treatment with the corresponding ligand ATRA on the proliferation and phenotype of platelet-derived growth factor (PDGF)-activated hepatic stellate cells (HSC).</p><p><b>METHODS</b>PDGF-activated hepatic stellate cells of rats were transfected with eukaryotic expression vector pCMV-script-RAR-beta, which was verified by western blot. The proliferation of transfected HSC was assayed by BrdU incorporation as well as MTT methods. Their phenotype (alpha-SMA and desmin) was observed by immunocytochemistry assay with image analysis and RAR-beta protein expression was detected by western blot.</p><p><b>RESULTS</b>Transfection of RAR-beta gene and treatment with ligand ATRA could increase the expression of RAR-beta protein for at least 144h and inhibit the proliferation and the expression of alpha-SMA and desmin in PDGF-activated HSC. Significant statistical differences were perceived comparing with sham-transfected, only-PDGF treated, non-ligand treated and irrelevant ligand-treated HSC.</p><p><b>CONCLUSIONS</b>Transfected with RAR-beta gene as well as using related ligand ATRA could suppress the proliferation and reverse the activation phenotype of activated HSC.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Phenotype
/
Physiology
/
Tretinoin
/
Platelet-Derived Growth Factor
/
Transfection
/
Cell Division
/
Blotting, Western
/
Receptors, Retinoic Acid
/
Cell Biology
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Hepatology
Year:
2002
Type:
Article
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